| Field | Specification |
|---|---|
| Target | |
| Alternative names | GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein; RAN; ARA24 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Ran GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACFI-18 is a rabbit monoclonal antibody that recognizes GTP-binding nuclear protein Ran in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 24.4 kDa, and the supplier reports an observed band at about 24 kDa.
Ran (RAN) is a small nuclear GTPase whose GTP-bound form is concentrated in the nucleus and GDP-bound form in the cytoplasm. This gradient directs importins and exportins to load and release cargo on the correct side of the nuclear envelope, and Ran-GTP also organizes spindle assembly during mitosis.
| Target | GTP-binding nuclear protein Ran (gene RAN; UniProt P62826, human) |
|---|---|
| Clone | ACFI-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.4 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human ran GTP-binding protein involved in nucleocytoplasmic transport.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places GTP-binding nuclear protein Ran in the nucleus, nucleus envelope, cytoplasm and melanosome. Tissue expression noted by UniProt (human): Expressed in a variety of tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
