| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ran-binding protein 9; RanBP9; BPM-L; BPM90; Ran-binding protein M; RanBPM; RanBP7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RANBP9 recombinant protein (Position: K348-E574). Human RANBP9 shares 95.6% amino acid (aa) sequence identity with mouse RANBP9. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ran-binding protein 9 (gene RANBP9) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 77.8 kDa, and the supplier reports an observed band at about 91 kDa.
RanBP9 (RanBPM) is a scaffold protein found in the nucleus and cytoplasm. It is a core component of the CTLH E3 ubiquitin ligase complex and interacts with many receptors and signaling proteins, with roles reported in cell spreading, actin organization and neuronal signaling.
| Target | Ran-binding protein 9 (gene RANBP9; UniProt Q96S59, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 77.8 kDa |
| Observed MW | 91 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human CCRF-CEM cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human RANBP9 fragment (Lys348–Glu574), expressed in E. coli. Sequence identity with the mouse ortholog: 95.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ran-binding protein 9 in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
