| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ran GTPase-activating protein 1; RanGAP1; KIAA1835; SD |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RanGAP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19R47 is a rabbit monoclonal antibody that recognizes ran GTPase-activating protein 1 (gene RANGAP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 63.5 kDa; the supplier reports observed bands at 64, 90 kDa.
RanGAP1 (RANGAP1) is the cytoplasmic GTPase-activating protein for Ran, converting Ran-GTP to Ran-GDP after transport complexes leave the nucleus. This step maintains the Ran gradient that drives nuclear import and export, and RanGAP1 is a classic SUMO-modified protein anchored at the nuclear pore through RanBP2.
| Target | Ran GTPase-activating protein 1 (gene RANGAP1; UniProt P46060, human) |
|---|---|
| Clone | 19R47 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.5 kDa |
| Observed MW | 64, 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ranGAP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ran GTPase-activating protein 1 in the cytoplasm, nucleus, nucleus envelope and chromosome. Tissue expression noted by UniProt (human): Highly expressed in brain, thymus and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
