| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 11A;Osteoclast differentiation factor receptor;ODFR;Receptor activator of NF-KB;CD265;TNFRSF11A;RANK; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human RANK, different from the related mouse sequence by seven amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-RANK/TNFRSF11A Antibody Picoband® catalog # A01064-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human RANK, different from the related mouse sequence by seven amino acids.
- Molecular weight context: reported MW: 80 kDa; calculated MW: 66034 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tumor necrosis factor receptor superfamily member 11A; Tumor necrosis factor receptor superfamily member 11A. Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. It is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, this gene is mapped to 18q22.1. Functional note: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. . Reported localization: Isoform 1: Cell membrane ; Single-pass type I membrane protein . Expression/tissue context: Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Research relevance and current trends
- Adapters: Researchers commonly examine how TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) relates to this theme using model systems and orthogonal readouts.
- Atherosclerosis: Researchers commonly examine how TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TNFRSF11A (Tumor necrosis factor receptor superfamily member 11A) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
