Anti-RANK/TNFRSF11A Antibody Picoband®

Overview

Anti-RANK/TNFRSF11A Antibody Picoband® is an antibody targeting TNFRSF11A. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 90 kDa; calculated MW: 66034 MW.

Boster Bio Anti-RANK/TNFRSF11A Antibody catalog # PA1382. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Target: TNFRSF11A — Tumor necrosis factor receptor superfamily member 11A
• Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 90 kDa; Calculated: 66034 MW

Specificity note: No cross reactivity with other proteins.

Biological background

Protein function (datasheet): Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. .

Scientific background (datasheet): Receptor Activator of Nuclear Factor K B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. RANKL (Receptor Activator for Nuclear Factor K B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.

Cellular localization (datasheet): Isoform 1: Cell membrane ; Single-pass type I membrane protein .

Tissue details (datasheet): Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Sequence similarities (datasheet): Contains 4 TNFR-Cys repeats.

Research relevance and current trends

• Commonly studied in contexts related to Adapters,Atherosclerosis,Cardiovascular,Cytokines,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction,Structures,TNF Superfamily,Transmembrane,Vascular Inflammation.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Cross-reactivity (datasheet): No cross-reactivity with other proteins
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.