| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; CD265; Tnfrsf11a; Rank |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse RANK/Tnfrsf11a recombinant protein (Position: Q30-R203). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor necrosis factor receptor superfamily member 11A (gene Tnfrsf11a) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 66.6 kDa, and the supplier reports an observed band at about 80 kDa.
RANK (TNFRSF11A) is the receptor for RANKL (TNFSF11). RANK signaling through TRAF6 activates NF-κB and NFATc1 and is essential for osteoclast formation and bone resorption. RANK also supports lymph node development, mammary gland development and dendritic cell function, and it is studied in bone biology, osteoporosis and bone metastasis.
| Target | Tumor necrosis factor receptor superfamily member 11A (gene Tnfrsf11a; UniProt O35305, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 66.6 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat thymus tissue, mouse thymus tissue, mouse RAW264.7 cells, mouse SP20 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells, RH-35 cells.
Immunogen
Recombinant mouse RANK/Tnfrsf11a fragment (Gln30–Arg203), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 11A in the cell membrane and membrane raft. Tissue expression noted by UniProt (mouse): Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jun Zhou et al. (2017) Effect of intervention initiation timing of pulsed electromagnetic field on ovariectomy-induced osteoporosis in rats. Bioelectromagnetics. 10.1002/bem.22059
