| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 5;EoCP;Eosinophil chemotactic cytokine;SIS-delta;Small-inducible cytokine A5;T cell-specific protein P228;TCP228;T-cell-specific protein RANTES;RANTES (3-68);RANTES (4-68);CCL5;D17S136E, SCYA5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human RANTES recombinant protein (Position: Y26-S91). Human RANTES shares 83.3% amino acid (aa) sequence identity with both mouse and rat RANTES. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets C-C motif chemokine 5 (gene CCL5) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 10.0 kDa, and the supplier reports an observed band at about 12 kDa.
CCL5, better known as RANTES, is a CC chemokine that draws monocytes, memory T cells and eosinophils into inflamed tissue. It engages several receptors, including CCR1, CCR3 and CCR5, and can also activate eosinophils and basophils. As a natural CCR5 ligand that suppresses HIV-1 entry, it is widely measured in studies of inflammation, allergy and viral infection.
| Target | C-C motif chemokine 5 (gene CCL5; UniProt P13501, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.0 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human NK-92 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human RANTES fragment (Tyr26–Ser91), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 83.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-C motif chemokine 5 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in the follicular fluid (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhen Li et al. (2010) Regulated upon activation normal T-cell expressed and secreted originating from the epididymis differentially associates with viable and defective spermatozoa. Fertility and Sterility. 10.1016/j.fertnstert.2010.01.053
Jin-Hua Wei et al. (2015) Different locations of RANTES and its receptors on mouse epididymal spermatozoa. Reproduction Fertility and Development. 10.1071/RD14231
Wen Liu et al. (2020) Blockage of C-C Chemokine Ligand 2 Alleviated the Appendicitis-Induced Injury in Rabbit Model. Journal of Interferon and Cytokine Research. 10.1089/jir.2020.0050
Yifan Wang et al. (2006) Triptolide inhibits CC chemokines expressed in rat adjuvant-induced arthritis. International Immunopharmacology. 10.1016/j.intimp.2006.07.029
Guimin Zhang et al. (2012) Distribution Change of Mast Cells in Human Nasal Polyps. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.22430
H. Zhao et al. (2013) The Significance for Chronic Rejection of Cardiac Allograft of Regulated Upon Activation Normal T-cell Cytokine and Its CCR5 Receptor. Transplantation Proceedings. 10.1016/j.transproceed.2012.03.065
