| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rap guanine nucleotide exchange factor 2; Cyclic nucleotide ras GEF; CNrasGEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA-GEF-1; Ras/Rap1-associating GEF-1; RAPGEF2; KIAA0313; NRAPGEP; PDZGEF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RAPGEF2 recombinant protein (Position: S1266-V1499). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets rap guanine nucleotide exchange factor 2 (gene RAPGEF2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 167.4 kDa, and the supplier reports an observed band at about 200–220 kDa.
RAPGEF2 (PDZ-GEF1) is a guanine nucleotide exchange factor that activates Rap1 and related small GTPases. It links cell surface receptors to Rap signaling and helps regulate cell junctions, neuronal migration and neurite growth.
| Target | Rap guanine nucleotide exchange factor 2 (gene RAPGEF2; UniProt Q9Y4G8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 167.4 kDa |
| Observed MW | 200–220 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human RAPGEF2 fragment (Ser1266–Val1499), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places rap guanine nucleotide exchange factor 2 in the cytoplasm, cell membrane, late endosome and cell junction. Tissue expression noted by UniProt (human): Expressed in primary neuronal and endocrine cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
