| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAS guanyl-releasing protein 1; Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein; RASGRP1; RASGRP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RASGRP1 recombinant protein (Position: R8-Q678). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets RAS guanyl-releasing protein 1 (gene RASGRP1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 90.4 kDa, and the supplier reports an observed band at about 100 kDa.
RasGRP1 is a guanine nucleotide exchange factor that switches on Ras in response to diacylglycerol and calcium, then feeds into the ERK/MAP kinase cascade. It couples T-cell and B-cell antigen receptors to Ras and is important for lymphocyte development, and it also shapes NK cell killing and mast cell responses, making it central to immune signaling studies.
| Target | RAS guanyl-releasing protein 1 (gene RASGRP1; UniProt O95267, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 90.4 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human 293T cells, human U-87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells, U2OS cells.
Immunogen
Recombinant human RASGRP1 fragment (Arg8–Gln678), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places RAS guanyl-releasing protein 1 in the cytoplasm, cell membrane, Golgi apparatus membrane and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
