| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras guanyl-releasing protein 3; Calcium and DAG-regulated guanine nucleotide exchange factor III; Guanine nucleotide exchange factor for Rap1; RASGRP3; GRP3; KIAA0846 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RASGRP3 recombinant protein (Position: K8-R607). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ras guanyl-releasing protein 3 (gene RASGRP3) in human samples and is validated for IHC and ELISA. The predicted molecular weight is 78.3 kDa.
RasGRP3 is a guanine nucleotide exchange factor that activates the small GTPases Ras and Rap1. Like other RasGRP family members, it responds to diacylglycerol and is studied in B-cell receptor signaling and growth factor pathways.
| Target | Ras guanyl-releasing protein 3 (gene RASGRP3; UniProt Q8IV61, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 78.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue, human rectum adenocarcinoma tissue, human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human RASGRP3 fragment (Lys8–Arg607), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
