Anti-RASSF2 Rabbit Monoclonal Antibody

SKU:BHA21010384
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Overview
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Anti-RASSF2 antibody (Rabbit monoclonal, clone 28R94, IgG). Validated for Western blot. Reactivity: Human. Recognizes ras association domain-containing protein 2. Observed band in Western blot: about 38 kDa.
Target RASSF2
clone number 28R94
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M04384-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RASSF2
Alternative names Ras association domain-containing protein 2; RASSF2; CENP-34; KIAA0168
UniProt # P50749
Host Rabbit
Clone 28R94
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human RASSF2
Molecular weight 37.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm. Chromosome, centromere, kinetochore.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M04384-1
Main SKU BHA21010384
Rabbit Monoclonal

Product Overview

Clone 28R94 is a rabbit monoclonal antibody that recognizes ras association domain-containing protein 2 (gene RASSF2) in human samples and is validated for Western blot. The predicted molecular weight is 37.8 kDa, and the supplier reports an observed band at about 38 kDa.

RASSF2 is a Ras-association domain protein that binds KRAS and is considered a potential tumor suppressor. It can promote apoptosis and cell cycle arrest and helps stabilize the Hippo pathway kinase MST2, so it is studied in Ras signaling and cancer biology.

Target Ras association domain-containing protein 2 (gene RASSF2; UniProt P50749, human)
Clone 28R94
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 37.8 kDa
Observed MW 38 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): Jurkat cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human RASSF2.

Reactivity Notes

The supplier lists reactivity with human. UniProt places ras association domain-containing protein 2 in the nucleus, cytoplasm and chromosome. Tissue expression noted by UniProt (human): Widely expressed with highest levels in brain, placenta, peripheral blood and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 37.8 kDa; observed band at about 38 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human RASSF2.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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