| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110;RB1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rb |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FCD-18 is a rabbit monoclonal antibody that recognizes retinoblastoma-associated protein (gene RB1) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 106.2 kDa, and the supplier reports an observed band at about 106 kDa.
The retinoblastoma protein (pRb, RB1) is a classic tumor suppressor that controls entry into S phase. In its underphosphorylated form it binds E2F transcription factors and recruits chromatin-modifying enzymes to silence cell cycle genes; phosphorylation by cyclin–CDK complexes releases E2F and allows DNA replication to begin. Loss of RB1 causes retinoblastoma, and pRb status is a standard readout in cell cycle and cancer research.
| Target | Retinoblastoma-associated protein (gene RB1; UniProt P06400, human) |
|---|---|
| Clone | FCD-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 106.2 kDa |
| Observed MW | 106 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human Jurkat cells, human MCF-7 cells, human 293T cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): rat lung tissue, human tongue cancer tissue, human renal cancer tissue, human kidney tissue, mouse spleen tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human rb.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places retinoblastoma-associated protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in the retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ke He et al. (2023) Integrative analysis of multi-omics data reveals inhibition of RB1 signaling promotes apatinib resistance of hepatocellular carcinoma. International Journal of Biological Sciences. 10.7150/ijbs.83862
Fangzheng Yin et al. (2020) Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone. Biomedicines. 10.3390/biomedicines8110472
Ruan Yunjun et al. (2014) Retarding the senescence of human vascular endothelial cells induced by hydrogen peroxide: effects of 17beta-estradiol (E2) mediated mitochondria protection. Biogerontology. 10.1007/s10522-014-9507-2
Xingsheng Hu et al. (2024) The expression and role of SUZ12 in lung adenocarcinoma. Cancer Medicine. 10.1002/cam4.70190
Wang Ping-Yu et al. (2010) Regulating A549 cells growth by ASO inhibiting miRNA expression. Molecular and Cellular Biochemistry. 10.1007/s11010-009-0380-2
