| Field | Specification |
|---|---|
| Target | |
| Alternative names | Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30; RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RBPJK Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEEF-18 is a rabbit monoclonal antibody that recognizes recombining binding protein suppressor of hairless (gene RBPJ) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 55.6 kDa, and the supplier reports an observed band at about 60 kDa.
RBPJ (also called CSL or RBP-Jκ) is the DNA-binding transcription factor at the heart of Notch signaling. Alone it represses Notch target genes by recruiting corepressor complexes, but when the Notch intracellular domain enters the nucleus it forms an activating complex that turns these genes on. RBPJ is therefore central to studies of cell fate decisions, development, stem cells and immunity.
| Target | Recombining binding protein suppressor of hairless (gene RBPJ; UniProt Q06330, human) |
|---|---|
| Clone | AEEF-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.6 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RBPJK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places recombining binding protein suppressor of hairless in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
