Anti-RCC1 Antibody Picoband®

Overview

Anti-RCC1 Antibody Picoband® is an antibody for RCC1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IHC-F, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: RCC1 (transient receptor potential cation channel subfamily C member 5); UniProt: P18754
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 45 kDa, calculated 91791 MW
• Applications: WB, IHC, IHC-F, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RCC1 Antibody Picoband® catalog # A02719-1.

Biological background

Biological context: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion.

Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein., tissue context: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole..

Common research applications

• Western blotting (WB): Compare RCC1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of RCC1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify RCC1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Multi-pass membrane protein.
• Tissue details: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
• Research category: Calcium Channels,Calcium Signaling,Metabolism,Neuroscience,Neurotransmission,Plasma Membrane,Signal Transduction,Signaling Pathway

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.