| Field | Specification |
|---|---|
| Target | |
| Alternative names | RING finger and CHY zinc finger domain-containing protein 1; Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; Zinc finger protein 363; p53-induced RING-H2 protein; hPirh2; RCHY1; ARNIP; CHIMP; PIRH2; RNF199; ZNF363 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RCHY1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26R35 is a rabbit monoclonal antibody that recognizes RING finger and CHY zinc finger domain-containing protein 1 (gene RCHY1) in human and mouse samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 30.1 kDa, and the supplier reports an observed band at about 30 kDa.
RCHY1 (Pirh2) is a RING-type E3 ubiquitin ligase that targets p53 for ubiquitination and proteasomal degradation, forming a feedback loop with p53, which induces its expression. It also acts on other substrates such as p73 and CDKN1B/p27 and has a role in ribosome-associated quality control, so it is studied in p53 regulation and cancer biology.
| Target | RING finger and CHY zinc finger domain-containing protein 1 (gene RCHY1; UniProt Q96PM5, human) |
|---|---|
| Clone | 26R35 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.1 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human RCHY1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places RING finger and CHY zinc finger domain-containing protein 1 in the nucleus, nucleus speckle and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
