| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Flotillin-1;FLOT1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human RCHY1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RCHY1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 26R35; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-RCHY1 Rabbit Monoclonal Antibody catalog # M04533-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: RCHY1 (Flotillin-1).
- Antibody format: Monoclonal; clone 26R35; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RCHY1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Reported cellular localization context: Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Specifically expressed in stromal cells of breast carcinomas.
Research relevance and current trends
- Research context keywords from the source record include: Cell Type Marker,Developmental Biology,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Lineage Markers,Lineage Specification,Neural Stem Cells,Neuron Marker,Neuroscience,Stem Cells,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate RCHY1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RCHY1 expression by Western blot in cell or tissue lysates, Localize RCHY1 by immunofluorescence/immunocytochemistry in cultured cells, Enrich RCHY1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 30 kDa; calculated MW: 47355 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 30 kDa
- Cellular localization (provided): Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Specifically expressed in stromal cells of breast carcinomas.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
