| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinol dehydrogenase 10; Short chain dehydrogenase/reductase family 16C member 4; RDH10; SDR16C4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RDH10 recombinant protein (Position: M1-I341). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets retinol dehydrogenase 10 (gene RDH10) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 38.1 kDa, and the supplier reports an observed band at about 38 kDa.
Retinol dehydrogenase 10 (RDH10) is a membrane-bound enzyme that oxidizes all-trans retinol to all-trans retinal using NADP+. This is the first and rate-limiting step in making retinoic acid in the embryo, so RDH10 is essential for retinoid signaling during development.
| Target | Retinol dehydrogenase 10 (gene RDH10; UniProt Q8IZV5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.1 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human RDH10 fragment (Met1–Ile341), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places retinol dehydrogenase 10 in the microsome membrane and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
