Anti-REC8 Antibody Picoband®

Overview

Anti-REC8 Antibody Picoband® is an antibody for REC8 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: REC8 (REC8 meiotic recombination protein); UniProt: O95072
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 63 kDa, calculated 56903 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-REC8 Antibody Picoband® catalog # A04915-2.

Biological background

Biological context: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Expression and localization notes: cellular localization: Nucleus. Chromosome. Centromere., tissue context: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa..

Common research applications

• Western blotting (WB): Compare REC8 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of REC8 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify REC8-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Meiotic recombination protein REC8 homolog is a protein that in humans is encoded by the REC8 gene. It is mapped to 14q12. This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus. Chromosome. Centromere.
• Tissue details: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.
• Research category: Vasculature

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.