| Field | Specification |
|---|---|
| Target | |
| Alternative names | RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor; REST; NRSF; XBR |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human REST / NRSF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17R95 is a rabbit monoclonal antibody that recognizes RE1-silencing transcription factor (gene REST) in human samples and is validated for Western blot. The predicted molecular weight is 121.9 kDa, and the supplier reports an observed band at about 160 kDa.
REST (also called NRSF) is a zinc-finger transcriptional repressor that binds the RE1/NRSE element found in many neuronal genes. In non-neuronal cells and neural progenitors it recruits the corepressors SIN3A and CoREST, which bring in histone deacetylases and LSD1 to keep neuronal genes silent, so REST is central to studies of neuronal identity, neurogenesis and brain disorders.
| Target | RE1-silencing transcription factor (gene REST; UniProt Q13127, human) |
|---|---|
| Clone | 17R95 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 121.9 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human REST / NRSF.
Reactivity Notes
The supplier lists reactivity with human. UniProt places RE1-silencing transcription factor in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in neurons of the prefrontal cortex, in hippocampal pyramidal neurons, dentate gyrus granule neurons and cerebellar Purkinje and granule neurons (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
