| Field | Specification |
|---|---|
| Target | |
| Alternative names | All-trans-retinol 13,14-reductase; All-trans-13,14-dihydroretinol saturase; RetSat; PPAR-alpha-regulated and starvation-induced gene protein; PPSIG |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RETSAT recombinant protein (Position: E35-K597). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects all-trans-retinol 13,14-reductase (gene RETSAT) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 66.8 kDa, and the supplier reports an observed band at about 67 kDa.
RETSAT is an ER-associated oxidoreductase that converts all-trans retinol into all-trans-13,14-dihydroretinol. It is thought to take part in vitamin A metabolism and has been linked to lipid metabolism, liver function and the response to oxidative stress.
| Target | All-trans-retinol 13,14-reductase (gene RETSAT; UniProt Q6NUM9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 66.8 kDa |
| Observed MW | 67 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human HUH-7 cells, human hepatocellular carcinoma tumor tissue (HCCT), rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human RETSAT fragment (Glu35–Lys597), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places all-trans-retinol 13,14-reductase in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
