| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oligoribonuclease, mitochondrial; RNA exonuclease 2 homolog; Small fragment nuclease; REXO2; SFN; SMFN |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human REXO2 recombinant protein (Position: Q59-D199). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects oligoribonuclease, mitochondrial (gene REXO2) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 26.8 kDa, and the supplier reports an observed band at about 27 kDa.
REXO2 is a 3′-to-5′ exonuclease found mainly in mitochondria that prefers very short RNA and DNA oligonucleotides, especially dinucleotides. It clears these small fragments produced by RNA decay, which helps maintain normal mitochondrial RNA and gene expression.
| Target | Oligoribonuclease, mitochondrial (gene REXO2; UniProt Q9Y3B8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.8 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human REXO2 fragment (Gln59–Asp199), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places oligoribonuclease, mitochondrial in the mitochondrion intermembrane space, mitochondrion matrix, mitochondrion and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in the heart and at lower levels in the lymph nodes, brain, lung, liver, spleen and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
