| Field | Specification |
|---|---|
| Target | |
| Alternative names | Reduced folate transporter; FOLT; Cyclic dinucleotide:anion antiporter SLC19A1; Folate:anion antiporter SLC19A1; Intestinal folate carrier 1; IFC-1; Placental folate transporter; Reduced folate carrier protein; RFC; hRFC; Reduced folate transporter 1; RFT-1; Solute carrier family 19 member 1; hSLC19A1; SLC19A1; FLOT1; RFC1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RFC/SLC19A1 recombinant protein (Position: V198-N231). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes reduced folate transporter (gene SLC19A1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 64.9 kDa, and the supplier reports an observed band at about 65 kDa.
The reduced folate carrier (RFC, SLC19A1) is the main route by which mammalian cells take up reduced folates such as 5-methyltetrahydrofolate, and it also transports antifolate drugs like methotrexate. More recently it was identified as an importer of the immune messenger 2′3′-cGAMP and related cyclic dinucleotides that activate STING.
| Target | Reduced folate transporter (gene SLC19A1; UniProt P41440, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 64.9 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human placenta tissue, human K562 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human RFC/SLC19A1 fragment (Val198–Asn231), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places reduced folate transporter in the cell membrane, apical cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Placenta, liver, and to a much smaller extent, in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
