| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-binding protein RFXANK; Ankyrin repeat family A protein 1; Regulatory factor X subunit B; RFX-B; Regulatory factor X-associated ankyrin-containing protein; RFXANK; ANKRA1; RFXB |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RFXANK recombinant protein (Position: E29-E260). Human RFXANK shares 85.1% amino acid (aa) sequence identity with mouse RFXANK. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA-binding protein RFXANK in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 34 kDa.
RFXANK is the ankyrin-repeat subunit of the RFX complex, which binds the X1 box of MHC class II promoters. Working with the coactivator CIITA, it is needed to switch on MHC class II genes, and RFXANK mutations cause bare lymphocyte syndrome type II, a form of combined immunodeficiency.
| Target | DNA-binding protein RFXANK (gene RFXANK; UniProt O14593, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.1 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human MCF-7 cells, human K562 cells, human PC-3 cells, rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human RFXANK fragment (Glu29–Glu260), expressed in E. coli. Sequence identity with the mouse ortholog: 85.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-binding protein RFXANK in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
