| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4; RACGAP1; KIAA1478 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RGAP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25R53 is a rabbit monoclonal antibody that recognizes rac GTPase-activating protein 1 (gene RACGAP1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 71.0 kDa, and the supplier reports an observed band at about 71 kDa.
RACGAP1 (MgcRacGAP) is a GTPase-activating protein that pairs with the kinesin MKLP1 to form centralspindlin at the spindle midzone. It recruits the RhoGEF ECT2 to activate RhoA for contractile ring formation and helps anchor the midbody during abscission, making it essential for cytokinesis.
| Target | Rac GTPase-activating protein 1 (gene RACGAP1; UniProt Q9H0H5, human) |
|---|---|
| Clone | 25R53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 71.0 kDa |
| Observed MW | 71 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human MCF-7 cells, human SIHA cells, human HeLa cells, human A549 cells, human Caco-2 cells, human U2OS cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human RGAP1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places rac GTPase-activating protein 1 in the nucleus, cytoplasm, cleavage furrow and midbody. Tissue expression noted by UniProt (human): Highly expressed in testis, thymus and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
