Anti-RGS6 Antibody Picoband®

Overview

Anti-RGS6 Antibody Picoband® is an antibody for RGS6 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: RGS6 (Protein phosphatase 1 regulatory subunit 14A); UniProt: P49758
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 54 kDa, calculated 16693 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RGS6 Antibody Picoband® catalog # A05753-1.

Biological background

Biological context: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

Expression and localization notes: cellular localization: Cytoplasm ., tissue context: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. ..

Common research applications

• Western blotting (WB): Compare RGS6 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of RGS6 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify RGS6-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Regulator of G-protein signaling 6 is a protein that in humans is encoded by the RGS6 gene. This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known.
• Cross reactivity: No cross-reactivity with other proteins
• Cellular localization: Cytoplasm .
• Tissue details: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
• Research category: Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.