| Field | Specification |
|---|---|
| Target | |
| Alternative names | GTP-binding protein Rheb; Ras homolog enriched in brain; RHEB; RHEB2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RHEB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19R40 is a rabbit monoclonal antibody that recognizes GTP-binding protein Rheb in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 20 kDa.
RHEB is a small GTPase that directly activates mTORC1 on the surface of lysosomes. Growth factor signaling through AKT inhibits the TSC complex, the GTPase-activating protein for RHEB, so RHEB stays GTP-bound and switches on mTORC1 to drive protein synthesis and cell growth. RHEB is central to studies of nutrient sensing, tuberous sclerosis and cancer.
| Target | GTP-binding protein Rheb (gene RHEB; UniProt Q15382, human) |
|---|---|
| Clone | 19R40 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.5 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RHEB.
Reactivity Notes
The supplier lists reactivity with human. UniProt places GTP-binding protein Rheb in the endomembrane system, lysosome membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
