| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAD9, HUS1, RAD1-interacting nuclear orphan protein 1; RAD9, RAD1, HUS1-interacting nuclear orphan protein; RHNO1; C12orf32; RHINO |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human RHNO1, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat RHNO1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 (gene RHNO1) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 26.7 kDa, and the supplier reports an observed band at about 40 kDa.
RHNO1 (also called RHINO) is a nuclear protein that binds the 9-1-1 checkpoint clamp (RAD9–HUS1–RAD1) and helps activate ATR signaling after DNA damage. It also recruits DNA polymerase theta to repair double-strand breaks by microhomology-mediated end joining during mitosis, and it is studied in DNA damage response research.
| Target | RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 (gene RHNO1; UniProt Q9BSD3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.7 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
Samples with a confirmed band (WB): human MOLT-4 cells, human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Synthetic peptide from the N-terminal region of human RHNO1. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Weakly expressed in testis, prostate, ovary, thymus and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bac Viet Le et al. (2026) Polθ activity modulates sensitivity to standard therapies in DNMT3A-deficient leukemia. Cell Reports Medicine. 10.1016/j.xcrm.2026.102687
