| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transforming protein RhoA;Rho cDNA clone 12;h12;RHOA;ARH12, ARHA, RHO12; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rho |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GOE-18 is a rabbit monoclonal antibody that recognizes transforming protein RhoA in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 21.8 kDa, and the supplier reports an observed band at about 22 kDa.
RhoA (RHOA) is a small GTPase that cycles between active GTP-bound and inactive GDP-bound states. Through effectors such as ROCK and mDia, active RhoA builds actin stress fibers and drives actomyosin contraction, controlling cell shape, adhesion, migration and cytokinesis. It is one of the most studied regulators of the actin cytoskeleton.
| Target | Transforming protein RhoA (gene RHOA; UniProt P61586, human) |
|---|---|
| Clone | GOE-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.8 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human rho.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transforming protein RhoA in the cell membrane, cytoplasm and cleavage furrow. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zheng Juyan et al. (2022) Midazolam Ameliorates Impairment of the Blood–Brain Barrier (BBB) Against LPS. Neurotoxicity Research. 10.1007/s12640-022-00508-4
Xiong Qian-Feng et al. (2018) GLP-1 attenuates Ang II-induced proliferation and migration in rat aorta smooth muscle cells via inhibition of the RhoA/ROCK2 signaling pathway. Pharmazie. 10.1691/ph.2018.8693
