| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho GTPase-activating protein 5; Rho-type GTPase-activating protein 5; p190-B; ARHGAP5; RHOGAP5 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RhoGAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A12 is a rabbit monoclonal antibody that recognizes rho GTPase-activating protein 5 (gene ARHGAP5) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 172.5 kDa, and the supplier reports an observed band at about 190 kDa.
Rho GTPase-activating protein 5 (ARHGAP5, also called p190B RhoGAP) turns off Rho family GTPases by promoting GTP hydrolysis. It is studied in cytoskeletal organization, cell adhesion and migration, and in development of tissues such as the mammary gland.
| Target | Rho GTPase-activating protein 5 (gene ARHGAP5; UniProt Q13017, human) |
|---|---|
| Clone | 25A12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 172.5 kDa |
| Observed MW | 190 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human rhoGAP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places rho GTPase-activating protein 5 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Detected in skin fibroblasts (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
