| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Receptor-interacting serine/threonine-protein kinase 1;2.7.11.1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP;RIPK1;RIP, RIP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human RIP |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-RIP RIPK1 Rabbit Monoclonal Antibody is an antibody targeting RIPK1. Common applications include WB, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IGF-18; isotype: Rabbit IgG; reactivity: Human; observed MW: 76 kDa; calculated MW: 75931 MW.
Boster Bio Anti-RIP RIPK1 Rabbit Monoclonal Antibody catalog # M00141. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: RIPK1 — Receptor-interacting serine/threonine-protein kinase 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IGF-18; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 76 kDa; Calculated: 75931 MW
Biological background
Protein function (datasheet): Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. .
Cellular localization (datasheet): Cytoplasm. Cell membrane .
Tissue details (datasheet): Produced by normal alveolar macrophages and granulocytes.
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cell Biology,Cell Death,Intracellular,Invasion/Microenvironment,Kinases,Protein Phosphorylation,Receptors,Ser/Thr Kinases,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
