| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-interacting serine/threonine-protein kinase 3;2.7.11.1;RIP-like protein kinase 3;Receptor-interacting protein 3;RIP-3;RIPK3;RIP3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects receptor-interacting serine/threonine-protein kinase 3 (gene RIPK3) in human samples and is validated for Western blot. The predicted molecular weight is 56.9 kDa; the supplier reports observed bands at 56 kDa, 77 kDa.
RIPK3 is a serine/threonine kinase that drives necroptosis, a regulated form of necrotic cell death. Activated through RIPK1, ZBP1 or TRIF, it forms the necrosome and phosphorylates the pseudokinase MLKL, which then damages the plasma membrane. RIPK3 is studied in inflammation, host defense against viruses and tissue injury.
| Target | Receptor-interacting serine/threonine-protein kinase 3 (gene RIPK3; UniProt Q9Y572, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 56.9 kDa |
| Observed MW | 56 kDa, 77 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): PANC cells, SW620 cells, SKOV-3 cells, MDA-MB-231 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human RIP3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places receptor-interacting serine/threonine-protein kinase 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in the pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qin Yang et al. (2019) Conditional knockout of TGF-βRII /Smad2 signals protects against acute renal injury by alleviating cell necroptosis, apoptosis and inflammation. Theranostics. 10.7150/thno.35686
Xue-Yu Sang et al. (2020) Regulators of calcineurin 1 deficiency attenuates tubulointerstitial fibrosis through improving mitochondrial fitness. FASEB Journal. 10.1096/fj.202000781RRR
Miao-Miao Jia et al. (2020) Jujuboside B promotes the death of acute leukemia cell in a RIPK1/RIPK3/MLKL pathway-dependent manner. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173041
