| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribonuclease K6; RNase K6; RNASE6; RNS6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RNASE6 recombinant protein (Position: P25-L150). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ribonuclease K6 (gene RNASE6) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 17.2 kDa, and the supplier reports an observed band at about 17 kDa.
Ribonuclease K6 (RNASE6) is an RNase A family enzyme that prefers uridine and cytosine. It is made by monocytes, macrophages and epithelial cells and kills many Gram-positive and Gram-negative bacterial species by damaging their membranes, an effect separate from its RNase activity, which makes it of interest in host defense, including the urinary tract.
| Target | Ribonuclease K6 (gene RNASE6; UniProt Q93091, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 17.2 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human RNASE6 fragment (Pro25–Leu150), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes ribonuclease K6 as secreted and also located in the lysosome and cytoplasmic granule. Tissue expression noted by UniProt (human): Highly expressed in spleen (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
