| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase Arkadia; RING finger protein 111; hRNF111; RING-type E3 ubiquitin transferase Arkadia; RNF111 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RNF111 recombinant protein (Position: F134-S994). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets E3 ubiquitin-protein ligase Arkadia (gene RNF111) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
Arkadia (RNF111) is a RING E3 ubiquitin ligase that boosts TGF-β/Nodal and BMP signaling. It does so by tagging negative regulators such as SMAD7, SKI and SKIL (SnoN) for proteasomal degradation, which strengthens SMAD2/SMAD3-dependent transcription, and it is required for mesoderm patterning in the early embryo.
| Target | E3 ubiquitin-protein ligase Arkadia (gene RNF111; UniProt Q6ZNA4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human RNF111 fragment (Phe134–Ser994), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 ubiquitin-protein ligase Arkadia in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Broadly expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
