| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase RNF115; RING finger protein 115; RING-type E3 ubiquitin transferase RNF115; Rab7-interacting RING finger protein; Rabring 7; Zinc finger protein 364; RNF115; ZNF364 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RNF115 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30R26 is a rabbit monoclonal antibody that recognizes E3 ubiquitin-protein ligase RNF115 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 33.7 kDa, and the supplier reports an observed band at about 37 kDa.
RNF115 (also called BCA2 or ZNF364) is a RING-type E3 ubiquitin ligase that can build both Lys48- and Lys63-linked chains. It helps route endocytosed receptors such as EGFR for lysosomal degradation and has been linked to autophagy, innate antiviral responses and cell proliferation, and it is often studied in breast cancer biology.
| Target | E3 ubiquitin-protein ligase RNF115 (gene RNF115; UniProt Q9Y4L5, human) |
|---|---|
| Clone | 30R26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.7 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RNF115.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 ubiquitin-protein ligase RNF115 in the cytoplasm, nucleus, endoplasmic reticulum and Golgi apparatus. Tissue expression noted by UniProt (human): Expressed at extremely low levels in normal breast, prostate, lung, colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
