| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; RNF43 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human RNF43 recombinant protein (Position: G24-Y197). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase RNF43 in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 85.7 kDa, and the supplier reports an observed band at about 86 kDa.
RNF43 is a transmembrane E3 ubiquitin ligase that dampens Wnt signaling by ubiquitinating Frizzled receptors, leading to their removal from the cell surface. Its activity is blocked when R-spondins bind LGR4–6 receptors, and together with its homolog ZNRF3 it is a key Wnt regulator in intestinal stem cells; loss-of-function mutations occur in several cancers.
| Target | E3 ubiquitin-protein ligase RNF43 (gene RNF43; UniProt Q68DV7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 85.7 kDa |
| Observed MW | 86 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human Caco-2 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human RNF43 fragment (Gly24–Tyr197), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places E3 ubiquitin-protein ligase RNF43 in the cell membrane, endoplasmic reticulum membrane and nucleus envelope. Tissue expression noted by UniProt (human): Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
