| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase RNF8; hRNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8; RNF8; KIAA0646 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RNF8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18R02 is a rabbit monoclonal antibody that recognizes E3 ubiquitin-protein ligase RNF8 in human and rat samples and is validated for Western blot. The predicted molecular weight is 55.5 kDa, and the supplier reports an observed band at about 60 kDa.
RNF8 is a RING E3 ubiquitin ligase that starts ubiquitin signaling at DNA double-strand breaks. It is recruited through its FHA domain to ATM-phosphorylated MDC1 and, with UBC13, builds Lys63-linked chains on chromatin that set the stage for RNF168 and the recruitment of 53BP1 and BRCA1 during repair.
| Target | E3 ubiquitin-protein ligase RNF8 (gene RNF8; UniProt O76064, human) |
|---|---|
| Clone | 18R02 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.5 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RNF8.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places E3 ubiquitin-protein ligase RNF8 in the nucleus, cytoplasm, midbody and chromosome. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
