| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ROBO1 recombinant protein (Position: F264-P897). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ROBO1 Antibody Picoband® is an antibody for ROBO1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ROBO1 (roundabout guidance receptor 1); UniProt: Q9Y6N7
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 250 kDa, calculated 180,930 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ROBO1 Antibody Picoband® catalog # A01530-2.
Biological background
Biological context: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein. Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane. Single-pass membrane protein., tissue context: Widely expressed, with exception of kidney..
Common research applications
- Western blotting (WB): Compare ROBO1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ROBO1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ROBO1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass type I membrane protein. Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane. Single-pass membrane protein.
- Tissue details: Widely expressed, with exception of kidney.
- Research category: Cancer,Cancer Metabolism,Cardiovascular,Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Redox Metabolism,Signal Transduction,Stem Cells
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
