| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Roundabout homolog 2, Roundabout Guidance Receptor 2 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-Robo2 (extracellular) Antibody is an antibody targeting Roundabout homolog 2, Roundabout Guidance Receptor 2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Roundabout homolog 2, Roundabout Guidance Receptor 2 (also reported as Roundabout homolog 2, Roundabout Guidance Receptor 2).
- Immunogen/epitope region: Extracellular..
- Homology note: Human, rat - identical. (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Robo2 (Roundabout homolog 2) is a member of the immunoglobulin superfamily and part of the ROBO receptor family, which is critical in the SLIT-ROBO signaling pathway. This receptor, characterized by extracellular immunoglobulin (Ig) and fibronectin type III (FNIII) domains, a transmembrane region, and an intracellular domain, mediates cellular migration, adhesion, and tissue remodeling during development and adulthood1. Robo2 is expressed in the nervous system, kidneys, pancreas, lungs, and retinal vasculature, contributing to organogenesis, angiogenesis, and immune modulation2,3.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR182.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR182; Negative control: BLP-NR182.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
