| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK; ROCK1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ROCK1 recombinant protein (Position: K601-R833). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets rho-associated protein kinase 1 (gene ROCK1) in human, monkey, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 158.2 kDa, and the supplier reports an observed band at about 160 kDa.
ROCK1 (Rho-associated protein kinase 1) is a serine/threonine kinase activated by GTP-bound RhoA. It phosphorylates targets such as myosin light chain, the myosin phosphatase subunit MYPT1 and LIM kinases to drive actomyosin contraction, stress fiber formation and cell migration, and ROCK inhibitors such as Y-27632 are standard research tools.
| Target | Rho-associated protein kinase 1 (gene ROCK1; UniProt Q13464, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 158.2 kDa |
| Observed MW | 160 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, monkey COS-7 cells, human A549-WT cells, human A549-ROCK1 KO cells, rat liver tissue, rat C6 cells, mouse liver tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human ROCK1 fragment (Lys601–Arg833), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places rho-associated protein kinase 1 in the cytoplasm, Golgi apparatus membrane, cell projection and cell membrane. Tissue expression noted by UniProt (human): Detected in blood platelets. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qin Dong et al. (2023) RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.17796
