| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; ROCK2; KIAA0619 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ROCK2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACDH-18 is a rabbit monoclonal antibody that recognizes rho-associated protein kinase 2 (gene ROCK2) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 160.9 kDa, and the supplier reports an observed band at about 161 kDa.
ROCK2 (Rho-associated protein kinase 2) is a RhoA-activated serine/threonine kinase closely related to ROCK1. It controls actomyosin contractility, focal adhesions and cell shape by phosphorylating regulators such as myosin light chain and MYPT1, and it is studied in smooth muscle contraction, fibrosis, neuronal outgrowth and immune regulation.
| Target | Rho-associated protein kinase 2 (gene ROCK2; UniProt O75116, human) |
|---|---|
| Clone | ACDH-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 160.9 kDa |
| Observed MW | 161 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human MCF-7 cells, human SH-SY5Y cells, rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ROCK2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places rho-associated protein kinase 2 in the cytoplasm, cell membrane and nucleus. Tissue expression noted by UniProt (human): Expressed in the brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongran Zhao et al. (2022) High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway. Investigative Ophthalmology & Visual Science. 10.1167/iovs.63.8.22
Han F et al. (2020) RhoA/Rock2/Limk1/cofilin1 pathway is involved in attenuation of neuronal dendritic spine loss by paeonol in the frontal cortex of D-galactose and aluminum‑induced Alzheimer's disease‑like rat model. ACTA Neurobiologiae Experimentalis. PMID: 32990282
Xiong Qian-Feng et al. (2018) GLP-1 attenuates Ang II-induced proliferation and migration in rat aorta smooth muscle cells via inhibition of the RhoA/ROCK2 signaling pathway. Pharmazie. 10.1691/ph.2018.8693
