| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase transmembrane receptor ROR2; Neurotrophic tyrosine kinase, receptor-related 2; ROR2; NTRKR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ROR2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23R31 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase transmembrane receptor ROR2 in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 104.8 kDa, and the supplier reports an observed band at about 100 kDa.
ROR2 is a receptor tyrosine kinase–like transmembrane protein that serves as a receptor or co-receptor for WNT5A in non-canonical Wnt signaling. It is important for skeletal development, including cartilage and growth plate formation, and mutations in ROR2 cause brachydactyly type B and recessive Robinow syndrome.
| Target | Tyrosine-protein kinase transmembrane receptor ROR2 (gene ROR2; UniProt Q01974, human) |
|---|---|
| Clone | 23R31 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 104.8 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human SH-SY5Y cells, human U2OS cells, human A431 cells, human SiHa cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ROR2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein kinase transmembrane receptor ROR2 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
