| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA polymerase II-associated protein 3; RPAP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RPAP3 recombinant protein (Position: R84-E604). Human RPAP3 shares 78.5% and 77.9% amino acid (aa) sequence identity with mouse and rat RPAP3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets RNA polymerase II-associated protein 3 (gene RPAP3) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 75.7 kDa, and the supplier reports an observed band at about 76 kDa.
RPAP3 is a co-chaperone that forms part of the R2TP complex with PIH1D1 and the AAA+ ATPases RUVBL1 and RUVBL2. Through its TPR domains it binds HSP90, linking this chaperone to the assembly of large complexes such as RNA polymerase II, snoRNPs and PIKK kinases.
| Target | RNA polymerase II-associated protein 3 (gene RPAP3; UniProt Q9H6T3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 75.7 kDa |
| Observed MW | 76 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human THP-1 cells, human K562 cells, human Caco-2 cells, rat PC-12 cells, rat NRK cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human RPAP3 fragment (Arg84–Glu604), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 78.5% and 77.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
