| Field | Specification |
|---|---|
| Target | |
| Alternative names | Large ribosomal subunit protein uL1; 60S ribosomal protein L10a; CSA-19; Neural precursor cell expressed developmentally down-regulated protein 6; NEDD-6; RPL10A; NEDD6 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RPL10A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29R56 is a rabbit monoclonal antibody that recognizes large ribosomal subunit protein uL1 (gene RPL10A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 24.8 kDa, and the supplier reports an observed band at about 25 kDa.
RPL10A (uL1) is a component of the large 60S ribosomal subunit, where it forms part of the L1 stalk that helps move deacylated tRNA out of the ribosome. Like other ribosomal proteins, it is used in studies of ribosome composition, translation and ribosome biogenesis.
| Target | Large ribosomal subunit protein uL1 (gene RPL10A; UniProt P62906, human) |
|---|---|
| Clone | 29R56 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.8 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RPL10A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places large ribosomal subunit protein uL1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
