| Field | Specification |
|---|---|
| Target | |
| Alternative names | Large ribosomal subunit protein eL13; 60S ribosomal protein L13; Breast basic conserved protein 1; RPL13; BBC1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RPL13 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27R88 is a rabbit monoclonal antibody that recognizes large ribosomal subunit protein eL13 (gene RPL13) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 24.3 kDa, and the supplier reports an observed band at about 26 kDa.
RPL13 (eL13) is a protein of the large 60S ribosomal subunit and contributes to the structure of the ribosome that carries out protein synthesis. Variants in RPL13 have been linked to a rare skeletal dysplasia, and the protein is often used in studies of ribosome composition and translation.
| Target | Large ribosomal subunit protein eL13 (gene RPL13; UniProt P26373, human) |
|---|---|
| Clone | 27R88 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.3 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RPL13.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places large ribosomal subunit protein eL13 in the cytoplasm. Tissue expression noted by UniProt (human): Higher levels of expression in benign breast lesions than in carcinomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
