| Field | Specification |
|---|---|
| Target | |
| Alternative names | Small ribosomal subunit protein eS19; 40S ribosomal protein S19; RPS19 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RPS19 recombinant protein (Position: M1-H145). Human RPS19 shares 99.3% amino acid (aa) sequence identity with both mouse and rat RPS19. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets small ribosomal subunit protein eS19 (gene RPS19) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry, immunoprecipitation and ELISA.
RPS19 (eS19) is a small-subunit ribosomal protein needed for pre-rRNA processing and maturation of the 40S subunit. It is the most frequently mutated gene in Diamond–Blackfan anemia, a congenital failure of red blood cell production, making it central to research on ribosome-related disorders.
| Target | Small ribosomal subunit protein eS19 (gene RPS19; UniProt P39019, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human RPS19 fragment (Met1–His145), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places small ribosomal subunit protein eS19 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
