| Field | Specification |
|---|---|
| Target | |
| Alternative names | Small ribosomal subunit protein eS19; 40S ribosomal protein S19; RPS19 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RPS19 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18R43 is a rabbit monoclonal antibody that recognizes small ribosomal subunit protein eS19 (gene RPS19) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 16.1 kDa, and the supplier reports an observed band at about 14 kDa.
RPS19 (eS19) is a small-subunit ribosomal protein needed for pre-rRNA processing and maturation of the 40S subunit. It is the most frequently mutated gene in Diamond–Blackfan anemia, a congenital failure of red blood cell production, making it central to research on ribosome-related disorders.
| Target | Small ribosomal subunit protein eS19 (gene RPS19; UniProt P39019, human) |
|---|---|
| Clone | 18R43 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.1 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human RPS19.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places small ribosomal subunit protein eS19 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Higher level expression is seen in the colon carcinoma tissue than normal colon tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
