| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RRM2; RR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human RRM2 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEAE-18 is a rabbit monoclonal antibody that recognizes ribonucleoside-diphosphate reductase subunit M2 (gene RRM2) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 44.9 kDa, and the supplier reports an observed band at about 45 kDa.
RRM2 is the small subunit of ribonucleotide reductase in proliferating cells, providing the tyrosyl radical the enzyme needs to make deoxyribonucleotides. Its levels peak in S phase and control the supply of dNTPs for DNA replication, making it a well-studied target of hydroxyurea and a marker of proliferation.
| Target | Ribonucleoside-diphosphate reductase subunit M2 (gene RRM2; UniProt P31350, human) |
|---|---|
| Clone | AEAE-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.9 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human SIHA cells, human U251 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human RRM2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ribonucleoside-diphosphate reductase subunit M2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanshan Ge et al. (2018) CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells. International Journal of Oncology. 10.3892/ijo.2018.4651
