| Field | Specification |
|---|---|
| Target | |
| Alternative names | RUN and FYVE domain-containing protein 1; FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12; RUFY1; RABIP4; ZFYVE12 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RUFY1 recombinant protein (Position: L20-Q650). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes RUN and FYVE domain-containing protein 1 (gene RUFY1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 79.8 kDa, and the supplier reports an observed band at about 80 kDa.
RUFY1 is an endosomal adaptor that carries an N-terminal RUN domain and a C-terminal FYVE domain, which binds phosphatidylinositol 3-phosphate. As an effector of the small GTPases RAB4 and RAB14, it supports endosome tethering and fusion and uses the dynein-dynactin motor to help retrieve cargo from endosomes back to the trans-Golgi network.
| Target | RUN and FYVE domain-containing protein 1 (gene RUFY1; UniProt Q96T51, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 79.8 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human RUFY1 fragment (Leu20–Gln650), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places RUN and FYVE domain-containing protein 1 in the early endosome membrane. Tissue expression noted by UniProt (human): Broadly expressed, with highest levels in lung, testis, kidney and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
