| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mucin-2; MUC-2; Intestinal mucin-2; MUC2; SMUC |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human S100A4 recombinant protein (Position: A2-K101). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-S100A4 Antibody Picoband® is an antibody for S100A4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: S100A4 (mucin 2, oligomeric mucus/gel-forming); UniProt: P26447
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 10-12 kDa, calculated 123799 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-S100A4 Antibody Picoband® catalog # A01217-2.
Biological background
Biological context: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Expression and localization notes: cellular localization: Secreted. In the intestine, secreted into the inner and outer mucus layers., tissue context: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder..
Common research applications
- Western blotting (WB): Compare S100A4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of S100A4 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify S100A4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: S100 calcium-binding protein A4 (S100A4) is a protein that in humans is encoded by the S100A4 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted. In the intestine, secreted into the inner and outer mucus layers.
- Tissue details: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
- Research category: Cancer,Cell Type Markers,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction,Tags & Cell Markers,Tumor Associated,Tumor Immunology,Tumor-Associated Antigens
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
