| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-A9;Calgranulin-B;Leukocyte L1 complex heavy chain;Migration inhibitory factor-related protein 14;MRP-14;p14;S100 calcium-binding protein A9;S100a9;Cagb, Mrp14; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein S100-A9 (gene S100a9) in mouse and rat samples and is validated for ELISA, flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 13.0 kDa, and the supplier reports an observed band at about 13–15 kDa.
S100A9 (also called calgranulin B or MRP14) is a calcium- and zinc-binding protein that forms calprotectin with S100A8 in neutrophils and monocytes. It supports phagocyte migration, NADPH oxidase activity and neutrophil degranulation, and once released it signals as an alarmin, making it a widely studied inflammatory marker.
| Target | Protein S100-A9 (gene S100a9; UniProt P31725, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 13.0 kDa |
| Observed MW | 13–15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HEPA1–6 cells.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Recombinant mouse S100A9 fragment (Ala2–Lys113), expressed in E. coli. Sequence identity with the human and rat orthologs: 59.8% and 78.6%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes protein S100-A9 as secreted and also located in the cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinbo Wei et al. (2025) Neutrophil Membrane-Engineered Extracellular Vesicles from Perivascular Adipose Tissue-Derived Stromal Cells for Atherosclerotic Lesion Resolution and Enhanced Vascular Graft Remodeling. Advanced Functional Materials. 10.1002/adfm.202503873
Liu Dan-Hua et al. (2025) Pulmonary mitochondrial DNA release and activation of the cGAS-STING pathway in Lethal Stx12 knockout mice. Cell Communication and Signaling. 10.1186/s12964-025-02141-y
Shuyin Duan et al. (2024) Single-cell RNA sequencing of estrual mice reveals PM2.5-induced uterine cell heterogeneity and reproductive toxicity. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2024.116968
Qing-Quan Liu et al. (2017) Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats. Oncotarget. 10.18632/oncotarget.18584
Deqiao Qin et al. (2025) Myricetin Attenuates IMQ-Induced Psoriatic Inflammation Through Multi-Target Modulation: Evidence from Network Pharmacology and Experimental Validation. Pharmaceuticals. 10.3390/ph18121802
Chen-Meng Qiao et al. (2024) Mechanism of S100A9-mediated astrocyte activation via TLR4/NF-κB in Parkinson’s disease. International Immunopharmacology. 10.1016/j.intimp.2024.113938
Lucibello Giulia et al. (2025) Harnessing Distinct Tissue-Resident Immune Niches via S100A9/TLR4 Improves Ketone, Lipid, and Glucose Metabolism. Endocrinology. 10.1210/endocr/bqaf131
