| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-A9;Calgranulin-B;Calprotectin L1H subunit;Leukocyte L1 complex heavy chain;Migration inhibitory factor-related protein 14;MRP-14;p14;S100 calcium-binding protein A9;S100A9;CAGB, CFAG, MRP14; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human S100A9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOOH-19 is a rabbit monoclonal antibody that recognizes protein S100-A9 (gene S100A9) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 13.2 kDa, and the supplier reports an observed band at about 14 kDa.
S100A9 (also called calgranulin B or MRP14) is a calcium- and zinc-binding protein that forms calprotectin with S100A8 in neutrophils and monocytes. It supports phagocyte migration, NADPH oxidase activity and neutrophil degranulation, and once released it signals as an alarmin, making it a widely studied inflammatory marker.
| Target | Protein S100-A9 (gene S100A9; UniProt P06702, human) |
|---|---|
| Clone | AOOH-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 13.2 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human S100A9.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes protein S100-A9 as secreted and also located in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Calprotectin (S100A8/9) is predominantly expressed in myeloid cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
